The short begins with Mabel filming Dipper and making it look like she's squishing her brother's head with her fingers. After telling Mabel to stop, Dipper tells the viewer that he'll be investigating Gravity Falls anomaly #23, "Grunkle Stan's Tattoo." He then points out a poster board of evidence and says that he's going to find out what the tattoo is. Mabel then says: "Right after another exciting episode of: What's under Mabel's bandage!" and starts to remove a pink bandage on her leg as Dipper shouts in disgust.
In the next scene, Dipper and Soos discuss a plan to turn up the heat on the thermostat and drive Stan to take off his undershirt. In the gift shop, Soos suggest to Stan that they work without their shirts on, to which he responds by saying he'll pay Soos to put his shirt back on and leaving to pull his eyeballs out. After Stan leaves, Soos begins to play with his belly fat.
Then, Dipper attempts "Plan B" and tries to see the tattoo by claiming there is poison oak on Stan's shoulder and that he wants to scratch it. Stan slaps Dipper's hand away and says that if he wants to see his tattoo he'll have to try harder than that. Dipper says smugly that he said he didn't have a tattoo. Stan says:"I don't. But you do!" He then pulls out a marker and forcibly writes "GOOBER" on Dipper's forehead.
Finally, Dipper says he's going to get a look at the tattoo while Stan is in the shower. He enters the bathroom only to find that Stan is fully clothed. Stan says: "You're never gonna see it kid. Never. Gonna. See it!" He then lunges for the camera. The short concludes with Dipper hiding from an angry Stan on the roof.
- Dipper knows about Stan's burn mark (but he thinks it is a tattoo).
- Stan denies the existence of his "tattoo."
- Dipper's birthmark which was first seen in "Double Dipper," makes another appearance.
- Mabel wears her mouse cursor sweater first seen in "Bottomless Pit!."
- The post it note reminding Dipper when to return a book to the library has the memo TK618.
- In an image on the Stan's Tattoo board, there is a text about secret society symbols that reads,
SECRET SOCIETY SYMBOLS
FIG. A Various Secret Society Symbols
Secret societies are (typically) fraternal orders that have been associated with everything from controlling international government to forbidden rituals to being a bunch of old guys playing gin rummy and complaining about their wives. They feature strange symbols like the ones you see above. One particularly sinister, mysterious order features a eye with a red x through it. Many rumors have flown around about these organizations, but here are some stone cold facts about secret societies. All secret societies worship a hyper-intelligent barn owl named Duane "The Enforcer" Roosevelt.
Most meetings consist of saying the secret oath, dressing in robes, hooting, gluing owl feathers to their skin, and devouring gophers whole in honor of their beloved barn owl overload. But not all secret societies worship barn owls, some of them worship western screeching owls. Also: Other owl-related jokes. In addition: Owls, owls, owls, owls, owls. And remember, when you don’t know where else to turn just ask yourself: "What would Duane Do?" Which is: Use your creepy heart-shaped face to see your prey, swoop down from a tree, eat an adult male vole, then cough up his bones into a disgusting ball that kids will later open in a high school biology class.
- At the end of the short another section of the hidden page was revealed. The cryptogram made by the red numbers decodes to "I WAS SO."
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